The Florida Central Railroad Company is one of several short line railroads run by Regional Rail, LLC. It runs from downtown Orlando northwest to Apopka and Tavares with a branch from Toronto to Ocoee and Winter Garden and branches from Tavares to Umatilla and Sorrento. The Florida Central connects with the Central Florida Rail Corridor in downtown Orlando, Florida and has trackage rights on the CFRC from there south to Taft Yard where they interchange with CSX Transportation. The railroad is based out of the Plymouth freight station.

The St. Johns and Lake Eustis Railway was a historic railroad in northern Central Florida. It ran from Astor on the St. Johns River south to Fort Mason and along Lake Eustis to Tavares and Lane Park. It also had track from Fort Mason to Leesburg, where it connected to the Florida Southern Railway.