State Road 24 is an east–west state highway that runs between Cedar Key on the Gulf of Mexico and Waldo, Florida, at US 301. State Road 24 runs along a former branch of the Seaboard Air Line Railroad. This branch was originally owned by the Florida Railway and Navigation Company, and used to lead to ferries to both Pensacola, Florida and New Orleans, Louisiana. It also passes through Rosewood, site of the infamous 1923 massacre.

Cedar Key is a city in Levy County, Florida, United States. As of the 2020 census, its population was 687, down from 702 at the 2010 census. It is part of the Gainesville, Florida Metropolitan Statistical Area. The Cedar Keys are a cluster of islands near the mainland. Most of the developed area for the City of Cedar Key has been on Way Key since the end of the 19th century. The Cedar Keys are named for the eastern red cedar Juniperus virginiana, once abundant in the area.