Gel electrophoresis
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Gel electrophoresis is an electrophoresis method for separation and analysis of biomacromolecules and their fragments, based on their size and charge through a gel. It is used in clinical chemistry to
Gel electrophoresis apparatus – an agarose gel is placed in this buffer-filled box and an electric current is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the positively charged anode (red wire). This occurs because phosphate groups found in the DNA fragments possess a negative charge which is repelled by the negatively charged cathode and are attracted to the positively charged anode.
Inserting the gel comb in an agarose gel electrophoresis chamber
Specific enzyme-linked staining: Glucose-6-Phosphate Dehydrogenase isoenzymes in Plasmodium falciparum infected Red blood cells
Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or with
Gel electrophoresis
…maintained by the addition of beta-mercaptoethanol or dithiothreitol. For a general analysis of protein samples, reducing PAGE is the most common form of protein electrophoresis. Denaturing conditions are necessary for proper estimation of molecular weight of RNA. RNA is able to form more intramolecular interactions than DNA…
Proteins separated by SDS-PAGE, Coomassie brilliant blue staining