Glover's Reef is a partially submerged atoll located off the southern coast of Belize, approximately 45 kilometres from the mainland. It forms part of the outermost boundary of the Belize Barrier Reef, and is one of its three atolls, besides Turneffe Atoll and Lighthouse Reef.

The Belize Barrier Reef is a series of coral reefs straddling the coast of Belize, roughly 300 metres (980 ft) offshore in the north and 40 kilometres (25 mi) in the south within the country limits. The Belize Barrier Reef is a 300-kilometre (190 mi) long section of the 900-kilometre (560 mi) Mesoamerican Barrier Reef System, which is continuous from Cancún on the north-eastern tip of the Yucatán Peninsula through the Riviera Maya and down to Honduras, making it the second largest coral reef system in the world after the Great Barrier Reef in Australia. It is Belize's top tourist destination, popular for scuba diving and snorkeling and attracting almost half of its 260,000 visitors. It is also vital to the country's fishing industry.