Great Lakes Brewing Company is a brewery and brewpub in Cleveland, Ohio. The first brewpub and microbrewery in the state, Great Lakes Brewing has been noted as important to Cleveland's local identity and as one of the initial forces behind the revival of the Ohio City neighborhood on the near West Side. In 2015, it was the 21st-largest craft brewery by volume and the 28th-largest overall brewery in the United States. The company was established in 1988 by brothers Patrick and Daniel Conway, both St. Edward High School graduates, in Cleveland's Ohio City neighborhood, located near St. Ignatius High School and the West Side Market. The brewpub and restaurant remain in their original locations, while production has expanded to adjacent properties.

Ohio City is one of the oldest neighborhoods in Cleveland, Ohio. It is located immediately west of the Cuyahoga River.