In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of a / v against a is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration a to the reaction
Hanes plot of a/v against a for Michaelis–Menten kinetics
In biochemistry, Michaelis–Menten kinetics, named after Leonor Michaelis and Maud Menten, is the simplest case of enzyme kinetics, applied to enzyme-catalysed reactions involving the transformation of
Hanes–Woolf plot
…in which the ratio of the initial substrate concentration a to the reaction velocity v is plotted against a . It is based on the rearrangement of the Michaelis–Menten equation shown below: where K m is the Michaelis constant and V is the limiting rate…
Curve of the Michaelis–Menten equation labelled in accordance with IUBMB recommendations
Semi-logarithmic plot of Michaelis–Menten data
The reaction changes from approximately first-order in substrate concentration at low concentrations to approximately zeroth order at high concentrations.