Hybridoma technology
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Hybridoma technology is a method for producing large quantities of monoclonal antibodies by fusing antibody producing B cells with myeloma cells. This creates hybrid cells, hybridomas, that produce th
Hybridoma cells grown in tissue culture. The image shows a single clone of cells each of which is producing large amounts of a specific monoclonal antibody which the cells secrete and which can be readily purified from the culture media.
Monoclonal antibody
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A monoclonal antibody is an antibody produced from a cell lineage made by cloning a unique white blood cell. All subsequent antibodies derived this way trace back to a unique parent cell.
Hybridoma technology
…Hybridoma technology is a method for producing large quantities of monoclonal antibodies by fusing antibody producing B cells with myeloma cells (cancerous B cells). This creates hybrid cells, hybridomas, that produce the antibody from their…
Looking at slides of cultures of cells that make monoclonal antibodies
Monoclonal antibodies can be grown in unlimited quantities in flasks.
Hand-filling wells with a liquid for a research test. This test involves preparation of cultures in which hybrids are grown in large quantities to produce desired antibody. This is effected by fusing a myeloma cell and a mouse lymphocyte to form a hybrid cell (hybridoma).
Bathing prepared slides in a solution