Interstate 75 (I-75) is a part of the Interstate Highway System that runs from the Hialeah–Miami Lakes border, a few miles northwest of Miami, to Sault Ste. Marie in the Upper Peninsula of Michigan. I-75 begins its national northward journey near Miami, running along the western parts of the Miami metropolitan area before traveling westward across Alligator Alley, resuming its northward direction in Naples, running along Florida's Gulf Coast, and passing the cities of Fort Myers, Punta Gorda, Venice, and Sarasota. The freeway passes through the Tampa Bay area before turning inward toward Ocala, Gainesville, and Lake City before leaving the state and entering Georgia. I-75 runs for 471 miles (758 km) in Florida, making it the longest Interstate in the state and also the longest in any state east of the Mississippi River. The Interstate's speed limit is 70 mph (110 km/h) for its entire length in Florida.

Interstate 4 (I-4) is an Interstate Highway located entirely within the US state of Florida, maintained by the Florida Department of Transportation (FDOT). Spanning 132.30 miles (212.92 km) along a generally southwest–northeast axis, I-4 is entirely concurrent with State Road 400 (SR 400). In the west, I-4 begins at an interchange with I-275 in Tampa. I-4 intersects with several major expressways as it traverses Central Florida, including US Highway 41 (US 41) in Tampa; US 301 near Riverview; I-75 near Brandon; US 98 in Lakeland; US 27 in unincorporated Davenport; US 192 in Celebration; Florida's Turnpike in Orlando; and US 17 and US 92 in multiple junctions. In the east, I-4 ends at an interchange with I-95 in Daytona Beach, while SR 400 continues for roughly another four miles (6.4 km) and ends at an intersection with US 1 on the city line of Daytona Beach and South Daytona.