Isabella of Portugal was the empress consort of her husband Charles V, Holy Roman Emperor, King of Spain, Archduke of Austria, and Duke of Burgundy. She was Queen of Spain and Germany, and Lady of the Netherlands from 10 March 1526 until her death in 1539, and became Holy Roman Empress and Queen of Italy in February 1530. She acted as regent of Spain during her husband's long absences.

El Escorial, or the Royal Site of San Lorenzo de El Escorial, or Monasterio de El Escorial, is a historical residence of the King of Spain located in the town of San Lorenzo de El Escorial, 2.06 kilometres (1.28 mi) up the valley from the town of El Escorial and about 45 kilometres (28 mi) northwest of the Spanish capital Madrid. Built between 1563 and 1584 by order of King Philip II, El Escorial is the largest Renaissance building in the world. It is one of the Spanish royal sites and functions as a monastery, basilica, royal palace, pantheon, library, museum, university, school, and hospital.