Jesup is a city in Wayne County, Georgia, United States. The population was 9,809 at the 2020 census. The city is the county seat of Wayne County.

U.S. Highway 25 (US 25) is a United States Numbered Highway that travels from Brunswick, Georgia, to the Kentucky–Ohio state line, where Covington, Kentucky, meets Cincinnati, Ohio, at the Ohio River. In the U.S. state of Georgia, US 25 is as a 190.0-mile-long (305.8 km) highway that travels south to north in the eastern part of the state, near the Atlantic Ocean, serving Statesboro and the Brunswick and Augusta metropolitan areas on its path from Brunswick to South Carolina at the Savannah River. Its routing travels through portions of Glynn, Wayne, Long, Tattnall, Evans, Bulloch, Jenkins, Burke, and Richmond counties.