John Biddle was an American military officer, politician, and businessman. He served as a delegate to the United States Congress from the Michigan Territory, as the speaker of the Michigan House of Representatives, and as mayor of Detroit.

The Biddle family of Philadelphia, Pennsylvania is an Old Philadelphian family descended from English immigrants William Biddle (1630–1712) and Sarah Kempe (1634–1709), who arrived in the Province of New Jersey in 1681. Quakers, they had emigrated from England in part to escape religious persecution. Having acquired extensive rights to more than 43,000 acres (170 km2) of lands in West Jersey, they settled first at Burlington, a city which developed along the east side of the Delaware River.