The John F. Kennedy Memorial Bridge is a six-lane, single-deck cantilever bridge that carries southbound Interstate 65 across the Ohio River, connecting Louisville, Kentucky and Jeffersonville, Indiana. The main span is 700 feet (213 m) and the bridge has a total length of 2,498 feet (761 m). The span carries six southbound lanes. It is named after U.S. President John F. Kennedy.

Interstate 65 (I-65) is a major north–south Interstate Highway in the central United States. As with most primary Interstates ending in 5, it is a major crosscountry, north–south route, connecting between the Great Lakes and the Gulf of Mexico. Its southern terminus is located at an interchange with I-10 in Mobile, Alabama, and its northern terminus is at an interchange with US 12 (US 12) and US 20 in Gary, Indiana, just southeast of Chicago. I-65 connects several major metropolitan areas in the Midwest and Southern US. It connects the four largest cities in Alabama: Mobile, Montgomery, Birmingham, and Huntsville. It also serves as one of the main north–south routes through Nashville, Tennessee; Louisville, Kentucky; and Indianapolis, Indiana, each a major metropolitan area in its respective state.