John Roy Hooper was an English churchman, Anglican Bishop of Gloucester, later of Worcester and Gloucester, a Protestant reformer and a Protestant martyr. A proponent of the English Reformation, he was executed for heresy by burning during the reign of Queen Mary I.

Mary I, also known as Mary Tudor, and as "Bloody Mary" by her Protestant opponents, was Queen of England and Ireland from July 1553 and Queen of Spain and the Habsburg dominions as the wife of King Philip II from January 1556 until her death in 1558. She is best known for her vigorous attempt to reverse the English Reformation, which had begun during the reign of her father, King Henry VIII. Her attempt to restore to the Church the property confiscated in the previous two reigns was largely thwarted by Parliament, but during her five-year reign, Mary had over 280 religious dissenters burned at the stake in the Marian persecutions.