Kary Banks Mullis was an American biochemist. In recognition of his invention of the polymerase chain reaction technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year, his invention became a central technique in biochemistry and molecular biology, described by The New York Times as "highly original and significant dividing biology into the two epochs of before PCR and after PCR." Mullis was born in Lenoir, North Carolina, near the Blue Ridge Mountains, on December 28, 1944. His family had a background in farming in this rural area; as a child, Mullis said, he was interested in observing organisms in the countryside. He grew up in Columbia, South Carolina, where he attended Dreher High School, graduating in the class of 1962, his interest in chemistry started when he learned how to chemically synthesize and build solid-state fuel propulsion rockets as a high school student during the 1950s. He earned a B. S. in chemistry from the Georgia Institute of Technology in Atlanta in 1966, during which time he married his first wife and started a business.
He earned his Ph. D. in 1973 in biochemistry at the University of California, Berkeley, in J. B. Neilands' laboratory, which focused on synthesis and structure of bacterial iron transporter molecules; as a graduate student, he published a sole-author paper in Nature in the field of astrophysics. Following his graduation, Mullis completed postdoctoral fellowships in pediatric cardiology at the University of Kansas Medical Center and pharmaceutical chemistry at the University of California, San Francisco. After receiving his doctorate, Mullis left science to write fiction before accepting the University of Kansas fellowship. During his postdoctoral work, he managed a bakery for two years. Mullis returned to science at the encouragement of friend Thomas White, who helped Mullis land a position with the biotechnology company Cetus Corporation of Emeryville, California. Mullis worked as a DNA chemist at Cetus for seven years. After leaving Cetus in 1986, Mullis served as director of molecular biology for Xytronyx, Inc. in San Diego for two years.
While inventing a UV-sensitive ink at Xytronyx, he became skeptical of the existence of the ozone hole. Mullis served as a consultant for multiple corporations on nucleic acid chemistry. While writing a National Institutes of Health grant progress report on the development of a human immunodeficiency virus test for Specialty Labs, he became skeptical that HIV was the cause of acquired immunodeficiency syndrome. In 1992, Mullis founded a business to sell pieces of jewelry containing the amplified DNA of deceased famous people like Elvis Presley and Marilyn Monroe. In 1992 he founded Atomic Tags, in La Jolla, California. Atomic Tags sought to develop technology using atomic-force microscopy and bar-coded antibodies tagged with heavy metals to create multiplexed, parallel immunoassays. Mullis was a member of the USA Engineering Festival's Advisory Board. In 2014, he was named a distinguished researcher at the Children's Hospital Oakland Research Institute in Oakland, California. In 1983, Mullis was working for Cetus Corporation as a chemist.
Late one night while driving with his girlfriend, a chemist at Cetus, he had the idea to use a pair of primers to bracket the desired DNA sequence and to copy it using DNA polymerase. Cetus took Mullis off his usual projects to concentrate on PCR full-time. Mullis succeeded in demonstrating PCR December 16, 1983. In his Nobel Prize lecture, he remarked that the success did not make up for his girlfriend breaking up with him. "I was sagging. Neither Fred, empty Beck's bottles, nor the sweet smell of the dawn of the age of PCR could replace Jenny. I was lonesome." He received a $10,000 bonus from Cetus for the invention. Other Cetus scientists, including Randall Saiki and Henry Erlich, were placed on PCR projects to work on determining if PCR could amplify a specific human gene from genomic DNA. Saiki generated the needed data and Erlich authored the first paper to include utilization of the technique, while Mullis was still working on a paper that would describe PCR itself. Mullis' 1985 paper with R. K. Saiki and H. A. Erlich, "Enzymatic Amplification of β-globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia"—the polymerase chain reaction invention -- was honored by a Citation for Chemical Breakthrough Award from the Division of History of Chemistry of the American Chemical Society in 2017.
A drawback of the technique was that the DNA polymerase in the reaction was destroyed by the high heat used at the start of each replication cycle and had to be replaced. In 1986, Saiki started to use Thermophilus aquaticus DNA polymerase to amplify segments of DNA; the Taq polymerase was heat resistant and only need to be added to the reaction once, making the technique more affordable and subject to automation. This modification of Mullis' invention revolutionized biochemistry, molecular biology, genetics and forensics. Mullis invented a UV-sensitive plastic that changes color in response to light, he founded Altermune LLC in 2011 to pursue new ideas on the immune system. Mullis described the company's novel technology in a presentation: It is a method using specific synthetic chemical linkers to divert an immune response from its nominal target to something different which you would right now like to be temporar
"Shake" is a song written and recorded by Sam Cooke. It was recorded at the last recording session Cooke had before his death on December 11, 1964. In the U. S. the song became a posthumous Billboard, Top 10 hit for Cooke, peaking at number seven in February 1965, as well as peaking at number two for three weeks on the Cashbox R&B charts. The song was recorded by Eric Burdon and The Animals, Otis Redding and Tina Turner, Rod Stewart, The Supremes on We Remember Sam Cooke. Redding's version was elected to the "500 Songs that Shaped Rock and Roll " by the Rock and Roll Hall of Fame and Museum. Redding performed the song backed by Booker T. & the M. G.'s at the Monterey Pop Festival in June 1967. Recordings of the performance have been released by Rhino records. In 1966, the British TV show Ready Steady Goes Live, devoted a whole programme to a live performance by Redding, who covered many of Cooke's songs. One of the highlights was a version of "Shake" on which Redding was joined by Eric Burdon and Chris Farlowe.
The song was covered by the Small Faces. It was a prominent part of their early live repertoire and featured as the opening track on their debut album Small Faces, re-released on the Decca Anthology collection. A live version can be found on their 1999 album The BBC Sessions. Eddie and the Hot Rods released a version of the song as the B-side to their 1976 single, "Teenage Depression". Ian Moss released a version of "Shake" as the lead single from his sixth studio album, Soul on West 53rd; the song was sampled by rapper Game on his 2011 album, The R. E. D. Album
Óscar Antonio Ramírez Hernández, is a Costa Rican former footballer who played as a midfielder, was most the manager of the Costa Rica national team. During the first half of the 2010s, he managed Alajuelense in two separate stints. Regarded as the most successful manager in the club's history, he won five league titles. Shortly after his second departure, he was appointed as the head coach of the Costa Rican national team, he played for the two teams in his country and Saprissa, becoming a star and an idol for both teams' fans. He made his debut for Liga on 13 November 1983 against Ramonense and scored his first goal a week against Municipal San José. With Alajuelense he won four national championships during the 1980s and early 1990s, as well as a CONCACAF Champions Cup in 1986. During 1993's season, El Macho switched to Alajuela's arch-rival team Saprissa, causing a commotion on Liga's fans. With Saprissa, he won a total of three more national championships and two CONCACAF Champions Cup titles.
In 1995, he moved to hometown club Belén but returned to Saprissa in 1997. He retired in March 2000 when at second division Guanacasteca. Ramírez made his debut for Costa Rica in a February 1985 friendly match against El Salvador and earned a total of 75 caps, scoring 6 goals, he represented his country in 21 FIFA World Cup qualification matches and played at the 1990 FIFA World Cup held in Italy. He played at the 1991 and 1997 UNCAF Nations Cups as well as at the 1991 CONCACAF Gold Cup and the 1997 Copa América in Bolivia, he collected his final cap in an August 1997 World Cup qualifier against El Salvador. Scores and results list Costa Rica's goal tally first. After his retirement, Ramírez began working as Hernán Medford's assistant coach in Saprissa, winning in less than three years, a national championship, a UNCAF Cup title, a CONCACAF Champions Cup title, thus earning a berth at the FIFA Club World Championship Toyota Cup; as of 28 October 2006, the Costa Rican Football Federation announced that Medford and his coaching staff would take charge of the Costa Rica national football team.
Thus making him the new assistant coach for the Costa Rica national football team. In May 2010, Ramírez took charge of Alajuelense, assisted by his former World Cup teammate Mauricio Montero, he was voted Costa Rican manager of the year 2012, but resigned in January 2013 only to return at the helm in May 2013. On August 2015, Ramírez was appointed as Paulo Wanchope's assistant coach for the Costa Rica national team. However, a week after his appointment, Wanchope was involved in a fight in Panama and announced his departure from the national team. Ramírez was appointed as head coach a week after. Ramírez is married to Jeannette Delgado and they have four children. Óscar Ramírez at National-Football-Teams.com Profile - Alajuelense Óscar ¿qué vas hacer? - Nación
Sweet Revenge 2 is a South Korean television series starring Ahn Seo-hyun, Samuel and Ji Min-hyuk. It is the sequel to the 2017 web series Sweet Revenge, it aired on xtvN's Mondays and Tuesdays at 20:00 time slot from August 13 to October 9, 2018. Oh Ji-na discovers an application on her phone which takes revenge on anyone whose name is written in it. Ahn Seo-hyun as Oh Ji-na Samuel Kim as Seo Robin Ji Min-hyuk as Seo Jaeyi Kim Ji-young as Oh Sa-na Ji-na's little sister. Park Hee-jin as Kim Eun-hee Ji-na and Sa-na's motherSeo Yu-ri as Kim Seon-hee Eun-hee's sister. Ji-na and Sa-na's aunt. Sung Ji-ru as Oh Kang-dong The convenience store boss. Ji-na and Sa-na's father.??? as Robin Jaeyi's mother Hwang Tae-kwang as Robin & Jaeyi's dad Kim Ye-eun as Maeng Sa-rang??? as Go Jin-sang Park Dong-bin as Lee Dae-ro Jeong Jeong-ah as Jeong Jeong-ah Hwang Tae-gwang as Choi Kang Kim So Hee as Geum Soo-ji Song Soo-hyun as Bae Shin-ae Park Seo-yeon as Jeong Bo-ra Lee Ji-hyun as Ha Rok-hee Shin Jun-hang as Ahn Mi-nam Cho Young-sun as Na Na-ri Gu Ji-hye as Bo Ra-hae Park Si-an as Baek Joo-ri?? as Jo A-ra Jo Jeong-eun as Park Saem-na Park Ji-hoo as Lee Ha-yan Lee Ho-jin as Kim Soo-geun Hong Dae-wi as Park Su-ro Lee Jun-young as Kang Ha-jun Song Tae-min as Han Seon-su Lee Jae-baek as Ha Ji-man Chung Young-hyun as Ban Lee-deun??? as Lim Ba-leun??? as Lee Myung-tae??? as Heo Gi-chan??? as Song Do-min Shin Ji Hoon I as Cho Eun-yeon Ji Gun-woo as Ho Go-joon Shin Bong-sun Kwon Mina as herself Episodes 13 and 14 did not air on September 24 and 25 due to Chuseok.
Official website Sweet Revenge 2 at HanCinema
Bassam Tariq is an American film director and screenwriter born in Karachi, Pakistan. He co-directed and produced the Sundance-funded documentary These Birds Walk with Omar Mullick, he was named in Filmmaker's "25 New Faces of Independent Film" in 2012. Tariq's works aim to uncover the diversity of Muslim experience. In addition to filmmaking, his diverse projects include blogging and writing, co-founding a halal butcher shop, being a TED fellow. Tariq lives in New York City with his son. Tariq was born in Pakistan and moved to the U. S. with his family at a young age. He grew up in New York's Astoria neighborhood and moved to Houston at age 11. Tariq was the first person in his family to attend college, he received a Bachelor of Science in advertising from the University of Texas at Austin in 2008. While in college, Tariq took a class called “Creativity in American Culture” that inspired him to start making films, which at first were corporate videos, he free-lanced and filmed promotional videos for Celestica, The University of Texas at Austin, CBS News 11.
Tariq started filmmaking in college to earn extra money while getting his degree. He has produced feature video stories for Time, a short film for The New Yorker, co-directed a PSA to encourage vaccination against polio in Pakistan, he moved to New York again after college and worked in advertising as a copywriter at Saatchi & Saatchi. He worked as copywriter at other agencies, including RAPP and BBDO NY. In 2009, Tariq and his friend Aman Ali started. During Ramadan and Ali broke their fast at different mosques around New York each night of Ramadan, they shared their stories on a Tumblr blog. During the second year of the project, the men decided to visit 30 mosques in 30 different states during Ramadan, they continued the tour of mosques around the country the following year; as they explored the different communities of each mosque and Ali wrote about the diversity of America's Muslim population and the themes that affect the Muslim community as a whole. The blog has gotten extensive press coverage, the response has been positive.
The Huffington Post called the project "visually stunning," and more communities around the world have participated in a 30 mosques project. In 2013, Tariq co-directed; the film follows street children in Pakistan. When Tariq moved back to New York, he read about a humanitarian in Pakistan named Abdul Sattar Edhi, who started the first ambulance system in Pakistan in the 1950s and founded the Edhi Foundation. Tariq and Mullick wanted to make a film about him, but Edhi urged them to focus on the work his foundation does; as a result, the film follows a young runaway boy, who lives in a home for runaways that Edhi runs. The Sundance-funded These Birds Walk premiered at South by Southwest 2013 and opened in theaters across the US in November 2013; the film explores the struggles of street children in Karachi, Pakistan who are living the Edhi Foundation, a non-profit social welfare program, the sympathetic but reluctant ambulance driver who has to take them back to the homes they've run away from.
In 2014, Tariq co-founded a halal butcher shop in the East Village in New York with Khalid Latif and Russell Khan. The shop, Honest Chops, was started because of the founders' frustration with the lack of halal meat options in New York City; the butcher shop sources organic, humanely-raised animals from the Tri-State area and attempts to make the product accessible and affordable. A portion of the profits goes toward building social institutions in New York City; the shop distributes meat to soup kitchens, food pantries and families in need around major holidays. Tariq is a TED Fellow. In October 2014, he hosted a TED Talk titled "The beauty and diversity of Muslim life." The talk explores how his eclectic career reflects his perspective on what it means to be Muslim, he relates his disparate professions as a response to the complicated history that America has with diversity and people who oversimplify Muslim beliefs and communities. Tariq is a freelance copywriter and creative director in New York City.
The Sundance Institute awarded Tariq and Mullick the Art of Nonfiction fellowship January 2016. The Art of Nonfiction initiative expands the institute's support for documentaries that explore contemporary social issues. Bassam Tariq on IMDb
All About Miriam is the 1966 ninth studio album of Miriam Makeba Arrangements for the album were by Luchi DeJesus and Sivuca. Sivuca played guitar. Harold Dodson played bass, drummer was Leopoldo Flemming. "The Ballad of the Sad Young Men" 3:00 "Yetentu Tizaleny" 2:34 "Maria Fulô" baião 2:53 "I Think I Ought To" 2:11 "Click Song" 2:16 "To Love and Lose" 3:33 "Four-Letter Words" 2:16 "U Shaka" 2:47 "Mas Que Nada" samba 2:52 "Mommy, Mommy What Is Heaven Like?" 2:46 "Jol'inkomo" 2:59 "The Sound of a Drum" 3:00