La Crosse is a city in and the county seat of La Crosse County, Wisconsin, United States. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population was 52,680 as of the 2020 census. The city forms the core of the La Crosse–Onalaska metropolitan area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627.

Gundersen Health System is a comprehensive non-profit health system based in La Crosse, Wisconsin. The system includes multi-specialty group medical practices, a teaching hospital, regional community clinics, affiliate hospitals and clinics, behavioral health services, vision centers, pharmacies, and air and ground ambulances.