La Trobe Street is a major street and thoroughfare in the Melbourne central business district, Victoria, Australia. It runs roughly east–west and forms the northern boundary of the central business district. The street was laid out as an extension of the original Hoddle Grid in 1839 and was named after Charles La Trobe. La Trobe Street extends from Victoria Street in the east to Harbour Esplanade in the west.

The Melbourne central business district is the city centre and main urban area of the city of Melbourne, Victoria, Australia, centred on the Hoddle Grid, the oldest part of the city laid out in 1837, and includes its fringes. The Melbourne CBD is located mostly in the local government area of the City of Melbourne, which also includes some of inner suburbs adjoining the CBD, while a small section extends into the City of Port Phillip.