Lameroo is a town in the Murray Mallee region of South Australia. It is on the Mallee Highway and Pinnaroo railway line about 40 km west of the Victorian border, or 210 km east of Adelaide. It is primarily a service town for the surrounding rural areas, growing grain and sheep. Lameroo now includes the former settlements of Kulkami, Mulpata, Wirha and Gurrai, which were on the Peebinga railway line, and Wilkawatt, which was between Parrakie and Lameroo on the Pinnaroo railway.

The Murray Mallee is the grain-growing and sheep-farming area of South Australia. It is bounded to the north and west by the Murray River, called the "River Murray" in South Australia, to the east by the Victorian border, and extending about 50 km south of the Mallee Highway.