Lone Tree is a city in southeastern Johnson County, Iowa, United States. It is part of the Iowa City, Iowa Metropolitan Statistical Area. The population was 1,357 at the time of the 2020 census.

Johnson County is located in the U.S. state of Iowa. As of the 2020 census, the population was 152,854, making it the fourth-most populous county in Iowa. The county seat is Iowa City, home of the University of Iowa. Johnson County is included in the Iowa City metropolitan area, which is also included in the Cedar Rapids-Iowa City Corridor Combined Statistical Area.