Middle Tennessee is one of the three Grand Divisions of Tennessee that composes roughly the central portion of the state. It is delineated according to state law as 41 of the state's 95 counties. Middle Tennessee contains the state's capital and largest city, Nashville, as well as Clarksville, the state's fifth largest city, and Murfreesboro, the state's sixth largest city and largest suburb of Nashville. The Nashville metropolitan area, located entirely within the region, is the most populous metropolitan area in the state, and the Clarksville metropolitan area is the state's sixth most populous. Middle Tennessee is both the largest, in terms of land area, and the most populous of the state's three Grand Divisions.

The Tennessee Walking Horse or Tennessee Walker is a breed of gaited horse known for its unique four-beat running-walk and flashy movement. It was originally developed as a riding horse on farms and plantations in the American South. It is a popular riding horse due to its calm disposition, smooth gaits and sure-footedness. The Tennessee Walking Horse is often seen in the show ring, but is also popular as a pleasure and trail riding horse using both English and Western equipment. Tennessee Walkers are also seen in movies, television, and other entertainment.