Middle Tennessee is one of the three Grand Divisions of Tennessee that composes roughly the central portion of the state. It is delineated according to state law as 41 of the state's 95 counties. Middle Tennessee contains the state's capital and largest city, Nashville, as well as Clarksville, the state's fifth largest city, and Murfreesboro, the state's sixth largest city and largest suburb of Nashville. The Nashville metropolitan area, located entirely within the region, is the most populous metropolitan area in the state, and the Clarksville metropolitan area is the state's sixth most populous. Middle Tennessee is both the largest, in terms of land area, and the most populous of the state's three Grand Divisions.

Nashville is the capital and most populous city in the U.S. state of Tennessee and the county seat of Davidson County. Located in Middle Tennessee, it had a population of 689,447 at the 2020 U.S. census. Nashville is the 21st most populous city in the United States, and the fourth most populous city in the southeastern U.S. Located on the Cumberland River, the city is the center of the Nashville metropolitan area, and is one of the fastest growing in the nation.