Mississippi Highway 389 is a state highway that runs from south to north in the U.S. State of Mississippi. MS 389 currently exists in two sections. The southern section begins at MS 182 in Starkville. The road travels north out of the city, and crosses U.S. Route 82 and MS 15. North of Starkville, the route continues northwestwards and ends at the Oktibbeha–Clay county line south of Pheba. The northern section starts at MS 46 west of Montpelier, and it travels north to cross the Natchez Trace Parkway in Chickasaw County. MS 389 ends at MS 8 in Houston.

Mississippi Highway 182 designates the old two-lane highway for U.S. Route 82 (US 82) where the current US 82 occupies a new right-of-way. Some of the towns with a segment of MS 182 are Winona, Kilmichael, Eupora, Mayhew, Starkville, and Columbus.