Native American tribes in the U.S. state of Nebraska have been Plains Indians, descendants of succeeding cultures of indigenous peoples who have occupied the area for thousands of years. More than 15 historic tribes have been identified as having lived in, hunted in, or otherwise occupied territory within the current state boundaries.

The Omaha Reservation of the federally recognized Omaha tribe is located mostly in Thurston County, Nebraska, with sections in neighboring Cuming and Burt counties, in addition to Monona County in Iowa. As of the 2020 federal census, the reservation population was 4,526. The tribal seat of government is in Macy. The villages of Rosalie, Pender and Walthill are located within reservation boundaries, as is the northernmost part of Bancroft. Due to land sales in the area since the reservation was established, Pender has disputed tribal jurisdiction over it, to which the Supreme Court ruled unanimously in 2016 that "the disputed land is within the reservation’s boundaries."