Osaka University , abbreviated as Handai (阪大), is a national research university in Osaka, Japan. The university traces its roots back to Edo-era institutions Tekijuku (1838) and Kaitokudo (1724), and was officially established in 1931 as the sixth of the Imperial Universities in Japan, with two faculties: science and medicine. Following the post-war educational reform, it merged with three pre-war higher schools, reorganizing as a comprehensive university with five faculties: science, medicine, letters, law and economics, and engineering. After the merger with Osaka University of Foreign Studies in 2007, it became the largest national university in Japan by undergraduate enrollment.

Osaka is a designated city in the Kansai region of Honshu in Japan, and one of the three major cities of Japan (Tokyo-Osaka-Nagoya). It is the capital of and most populous city in Osaka Prefecture, and the third-most populous city in Japan, following the special wards of Tokyo and Yokohama. With a population of 2.7 million in the 2020 census, it is also the largest component of the Keihanshin Metropolitan Area, which is the second-largest metropolitan area in Japan and the 10th-largest urban area in the world with more than 19 million inhabitants.