Pennsylvania Route 216 is a 26-mile-long (42 km) state highway located in York County, Pennsylvania. The western terminus is at PA 116 near Hanover. The eastern terminus is at PA 24 in Winterstown. PA 216 is a two-lane undivided road that passes through rural areas in southern York County, serving Sticks and Glen Rock. The route intersects PA 516 in Sticks, PA 616 in Glen Rock, and Interstate 83 (I-83) near Loganville.

York County is a county in the Commonwealth of Pennsylvania, United States. As of the 2020 census, the population was 456,438. Its county seat is York. The county was created on August 19, 1749, from part of Lancaster County and named either after the Duke of York, an early patron of the Penn family, or for the city and county of York in England. The county is part of the South Central region of the state.