Polyacrylamide gel electrophoresis is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins
Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane
Two SDS-PAGE-gels after a completed run
SDS-PAGE
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SDS-PAGE is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa. The combined use
Polyacrylamide gel electrophoresis
…the protein-SDS (sodium dodecyl sulfate) complexes all have a similar mass-to-charge ratio). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight. At the pH at which gel electrophoresis is carried out the SDS…
Proteins of the erythrocyte membrane separated by SDS-PAGE according to their molecular masses
Sample combs with different numbers of pockets, each prong leaves a pocket in the gel when pulled out
Polymerised separating and stacking gel before removing the sample comb (white) between the spacers (black), in the stacking gel are small amounts of bromophenol blue for improved visibility, the separating gel is unstained
Electrophoresis chamber after a few minutes of electrophoresis. In the first pocket a size marker was applied with bromophenol blue, in the other pockets, the samples were added bromocresol green