The Portrait of Giovanna Tornabuoni is a painting by the Italian Renaissance painter Domenico Ghirlandaio, executed in 1488 and located in the Museo Thyssen-Bornemisza, Madrid. The portrait was commissioned by Lorenzo Tornabuoni after his wife's death in 1488 and includes many symbolic details.

Domenico di Tommaso Curradi di Doffo Bigordi, professionally known as Domenico Ghirlandaio, was an Italian Renaissance painter born in Florence. Ghirlandaio was part of the so-called "third generation" of the Florentine Renaissance, along with Verrocchio, the Pollaiolo brothers and Sandro Botticelli. Ghirlandaio led a large and efficient workshop that included his brothers Davide Ghirlandaio and Benedetto Ghirlandaio, his brother-in-law Bastiano Mainardi from San Gimignano, and later his son Ridolfo Ghirlandaio. Many apprentices passed through Ghirlandaio's workshop, including the famous Michelangelo. His particular talent lay in his ability to posit depictions of contemporary life and portraits of contemporary people within the context of religious narratives, bringing him great popularity and many large commissions.