The Prince-Bishopric of Würzburg was an ecclesiastical principality of the Holy Roman Empire located in Lower Franconia, west of the Prince-Bishopric of Bamberg. Würzburg had been a diocese since 743. As established by the Concordat of 1448, bishops in Germany were chosen by the canons of the cathedral chapter and their election was later confirmed by the pope. Following a common practice in Germany, the prince-bishops of Würzburg were frequently elected to other ecclesiastical principalities as well. The last few prince-bishops resided at the Würzburg Residence, which is one of the grandest Baroque palaces in Europe.

In the Holy Roman Empire, the German term Hochstift referred to the territory ruled by a bishop as a prince, as opposed to his diocese, generally much larger and over which he exercised only spiritual authority. The terms prince-bishopric and ecclesiastical principality are synonymous with Hochstift. Erzstift and Kurerzstift referred respectively to the territory (prince-archbishopric) ruled by a prince-archbishop and an elector-archbishop while Stift referred to the territory ruled by an imperial abbot or abbess, or a princely abbot or abbess. Stift was also often used to refer to any type of ecclesiastical principality.