The Princes in the Tower refers to the mystery of the fate of the deposed King Edward V of England and his younger brother Prince Richard of Shrewsbury, Duke of York, heirs to the throne of King Edward IV of England. The brothers were the only sons of the king by his queen, Elizabeth Woodville, living at the time of their father's death in 1483. Aged 12 and 9 years old, respectively, they were lodged in the Tower of London by their paternal uncle and England's regent, Richard, Duke of Gloucester, supposedly in preparation for Edward V's forthcoming coronation. Before the young king could be crowned, however, he and his brother were declared illegitimate. Gloucester ascended the throne as Richard III.

Edward V was King of England from 9 April to 25 June 1483. He succeeded his father, Edward IV, upon the latter's death. Edward V was never crowned, and his brief reign was dominated by the influence of his uncle and Lord Protector, the Duke of Gloucester, who deposed him to reign as King Richard III; this was confirmed by the Titulus Regius, an Act of Parliament which denounced any further claims through Edward IV's heirs by delegitimising Edward V and all of his siblings. This was later repealed by Henry VII, who wished to legitimise his reign by marrying Elizabeth of York, Edward's eldest sister.