Santa Cruz is the largest of the nine constituent departments of Bolivia, occupying about one-third (33.74%) of the country's territory. With an area of 370,621 km2 (143,098 sq mi), it is slightly smaller than Japan or the US state of Montana. It is located in the eastern part of the country, sharing borders in the north and east with Brazil and with Paraguay in the south.

Bolivia, officially the Plurinational State of Bolivia, is a landlocked country located in western-central South America. It is bordered by Brazil to the north and east, Paraguay to the southeast, Argentina to the south, Chile to the southwest, and Peru to the west. The seat of government and administrative capital is La Paz, which contains the executive, legislative, and electoral branches of government, while the constitutional capital is Sucre, the seat of the judiciary. The largest city and principal industrial center is Santa Cruz de la Sierra, located on the Llanos Orientales, a mostly flat region in the east of the country.