The Sasanian conquest of Jerusalem or Sasanian conquest of Palestine was a significant event in the Byzantine–Sasanian War of 602–628, having taken place in early 614. Amidst the conflict, Sasanian king Khosrow II had appointed Shahrbaraz, his spahbod, to lead an offensive into the Diocese of the East of the Byzantine Empire. Under Shahrbaraz, the Sasanian army had secured victories at Antioch as well as at Caesarea Maritima, the administrative capital of Palaestina Prima. By this time, the grand inner harbour had silted up and was useless, but the city continued to be an important maritime hub after Byzantine emperor Anastasius I Dicorus ordered the reconstruction of the outer harbour. Successfully capturing the city and the harbour had given the Sasanian Empire strategic access to the Mediterranean Sea. The Sasanians' advance was accompanied by the outbreak of a Jewish revolt against Heraclius; the Sasanian army was joined by Nehemiah ben Hushiel and Benjamin of Tiberias, who enlisted and armed Jews from across Galilee, including the cities of Tiberias and Nazareth. In total, between 20,000 and 26,000 Jewish rebels took part in the Sasanian assault on Jerusalem. By mid-614, the Jews and the Sasanians had captured the city, but sources vary on whether this occurred without resistance or after a siege and breaching of the wall with artillery. Following the Sasanians capture of Jerusalem tens of thousands of Byzantine Christians were massacred by the Jewish rebels.

The Byzantine–Sasanian War of 602–628 was the final and most devastating of the series of wars fought between the Byzantine Empire and the Persian Sasanian Empire. The previous war between the two powers had ended in 591 after Emperor Maurice helped the Sasanian king Khosrow II regain his throne. In 602 Maurice was murdered by his political rival Phocas. Khosrow declared war, ostensibly to avenge the death of the deposed emperor Maurice. This became a decades-long conflict, the longest war in the series, and was fought throughout the Middle East: in Egypt, the Levant, Mesopotamia, the Caucasus, Anatolia, Armenia, the Aegean Sea and before the walls of Constantinople itself.