Shrine of Our Lady of Guadalupe is a Catholic shrine located in La Crosse, Wisconsin. It is dedicated to the Blessed Virgin Mary under the title of Our Lady of Guadalupe. The 100-acre (0.40 km2) grounds include a visitors' center and outdoor devotional areas such as a rosary walk, Stations of the Cross, and a votive candle chapel. Mass and the sacrament of Penance are celebrated daily by Norbertine canons of St. Michael's Abbey.

La Crosse is a city in and the county seat of La Crosse County, Wisconsin, United States. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population was 52,680 as of the 2020 census. The city forms the core of the La Crosse–Onalaska metropolitan area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627.