Singapore competed at the 2016 Summer Olympics in Rio de Janeiro, Brazil, from 5 to 21 August 2016. This was the nation's sixteenth appearance at the Summer Olympics, except for two different editions. Singapore was part of the Malaysian team at the 1964 Summer Olympics in Tokyo, but did not attend at the 1980 Summer Olympics in Moscow, because of its support for the United States boycott.

The 2016 Summer Olympics, officially the Games of the XXXI Olympiad and officially branded as Rio 2016, were an international multi-sport event held from 5 to 21 August 2016 in Rio de Janeiro, Brazil, with preliminary events in some sports beginning on 3 August. Rio de Janeiro was announced as the host city at the 121st IOC Session in Copenhagen, Denmark, on 2 October 2009.