Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. It is usua
Equipment for running size-exclusion chromatography. The buffer is pumped through the column (left).
Agarose-based SEC columns used for protein purification on an AKTA FPLC machine
Multiangle light scattering
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Multiangle light scattering describes a technique for measuring the light scattered by a sample into a plurality of angles. It is used for determining both the absolute molar mass and the average size
Size-exclusion chromatography
…other techniques may be necessary to identify the contents of each fraction. It is also possible to analyze the eluent flow continuously with RI, LALLS, Multi-Angle Laser Light Scattering MALS, UV, and/or viscosity measurements. The elution volume (Ve) decreases roughly linear with the logarithm of the molecular hydrodynamic volume…
Zimm plot
MALS signals for polystyrene spheres
BSA Separation and MM distribution