The South Florida Railroad was a railroad from Sanford, Florida, to Tampa, Florida, becoming part of the Plant System in 1893 and the Atlantic Coast Line Railroad in 1902. It served as the southernmost segment of the Atlantic Coast Line's main line. The line remains in service today and is now part of the Central Florida Rail Corridor in the Orlando metro area. The rest of the line remains under the ownership of CSX Transportation as part of their A Line.

Sanford is a city and the county seat of Seminole County, Florida. It is located in Central Florida and its population was 61,051 as of the 2020 census. It is part of the Orlando–Kissimmee–Sanford Metropolitan Statistical Area.