The Stadt- und Pfarrkirche St. Marien zu Wittenberg is the civic church of the German town of Lutherstadt Wittenberg. The reformers Martin Luther and Johannes Bugenhagen preached there and the building also saw the first celebration of the mass in German rather than Latin and the first ever distribution of the bread and wine to the congregation – it is thus considered the mother-church of the Protestant Reformation. In 1996, it was inscribed on the UNESCO World Heritage List along with Castle Church of All Saints (Schlosskirche), the Lutherhaus, the Melanchthonhaus, and Martin Luther's birth house and death house in Eisleben, because of its religious significance and testimony to the lasting, global influence of Protestantism.

Wittenberg, is the fourth-largest town in Saxony-Anhalt, Germany. Wittenberg is situated on the River Elbe, 60 kilometers (37 mi) north of Leipzig and 90 kilometers (56 mi) south-west of Berlin, and has a population of 46,008 (2018).