Tempera, also known as egg tempera, is a permanent, fast-drying painting medium consisting of pigments mixed with a water-soluble binder medium, usually glutinous material such as egg yolk. Tempera also refers to the paintings done in this medium. Tempera paintings are very long-lasting, and examples from the first century AD still exist. Egg tempera was a primary method of painting until after 1500 when it was superseded by oil painting. A paint consisting of pigment and binder commonly used in the United States as poster paint is also often referred to as "tempera paint", although the binders in this paint are different from traditional tempera paint.

Among animals which produce eggs, the yolk is the nutrient-bearing portion of the egg whose primary function is to supply food for the development of the embryo. Some types of egg contain no yolk, for example because they are laid in situations where the food supply is sufficient or because the embryo develops in the parent's body, which supplies the food, usually through a placenta. Reproductive systems in which the mother's body supplies the embryo directly are said to be matrotrophic; those in which the embryo is supplied by yolk are said to be lecithotrophic. In many species, such as all birds, and most reptiles and insects, the yolk takes the form of a special storage organ constructed in the reproductive tract of the mother. In many other animals, especially very small species such as some fish and invertebrates, the yolk material is not in a special organ, but inside the egg cell.