The Tennessee Aquarium is a non-profit public aquarium located in Chattanooga, Tennessee, United States. It opened in 1992 on the banks of the Tennessee River in downtown Chattanooga, with a major expansion added in 2005. The aquarium, which has been accredited by the Association of Zoos and Aquariums (AZA) since 1993, is home to more than 12,000 animals representing almost 800 species.

Chattanooga is a city in, and the county seat of, Hamilton County, Tennessee, United States. It is located along the Tennessee River, and borders Georgia to the south. With a population of 181,099 in 2020, it is Tennessee's fourth-largest city and one of the two principal cities of East Tennessee, along with Knoxville. It anchors the Chattanooga metropolitan area, Tennessee's fourth-largest metropolitan statistical area, as well as a larger three-state area that includes Southeast Tennessee, Northwest Georgia, and Northeast Alabama.