The Tuolumne River flows for 149 miles (240 km) through Central California, from the high Sierra Nevada to join the San Joaquin River in the Central Valley. Originating at over 8,000 feet (2,400 m) above sea level in Yosemite National Park, the Tuolumne drains a rugged watershed of 1,958 square miles (5,070 km2), carving a series of canyons through the western slope of the Sierra. While the upper Tuolumne is a fast-flowing mountain stream, the lower river crosses a broad, fertile and extensively cultivated alluvial plain. Like most other central California rivers, the Tuolumne is dammed multiple times for irrigation and the generation of hydroelectricity.

Yosemite National Park is a national park in California. It is bordered on the southeast by Sierra National Forest and on the northwest by Stanislaus National Forest. The park is managed by the National Park Service and covers 759,620 acres in four counties – centered in Tuolumne and Mariposa, extending north and east to Mono and south to Madera. Designated a World Heritage Site in 1984, Yosemite is internationally recognized for its granite cliffs, waterfalls, clear streams, giant sequoia groves, lakes, mountains, meadows, glaciers, and biological diversity. Almost 95 percent of the park is designated wilderness. Yosemite is one of the largest and least fragmented habitat blocks in the Sierra Nevada.