U.S. Route 29 (US 29) is a major north–south route in the commonwealth of Virginia. It covers 248.0 miles (399.1 km) from the North Carolina border at the city of Danville to the Key Bridge in Washington DC. US 29 roughly bisects Virginia into eastern and western halves and, along with Interstate 81 (I-81) and US 11 in western Virginia and I-85/I-95 as well as US 1 farther east, provides one of the major north–south routes through the commonwealth.

Danville is an independent city in the Commonwealth of Virginia in the United States. The city is located in the Southside Virginia region and on the fall line of the Dan River. It was a center of tobacco production and was an area of Confederate activity during the American Civil War, due to its strategic location on the Richmond and Danville Railroad. In April 1865 it briefly served as the third and final capital of the Confederacy before its surrender later that year.