U4atac minor spliceosomal RNA is a ncRNA which is an essential component of the minor U12-type spliceosome complex. The U12-type spliceosome is required for removal of the rarer class of eukaryotic in
Predicted secondary structure and sequence conservation of U4atac
Spliceosome
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A spliceosome is a large ribonucleoprotein complex found primarily within the nucleus of eukaryotic cells. The spliceosome is assembled from small nuclear RNAs and numerous proteins. Small nuclear RNA
U4atac minor spliceosomal RNA
…a 3' Sm protein binding site which has been shown to be essential for splicing activity. U4atac is the functional analog of U4 spliceosomal RNA in the major U2-type spliceosomal complex. The Drosophila U4atac snRNA has an additional predicted 3' stem loop terminal to the Sm binding site…
Spliceosomal splicing cycle
Figure 1. Above are electron microscopy fields of negatively stained yeast (Saccharomyces cerevisiae) tri-snRNPs. Below left is a schematic illustration of the interaction of tri-snRNP proteins with the U4/U6 snRNA duplex. Below right is a cartoon model of the yeast tri-snRNP with shaded areas corresponding to U5 (gray), U4/U6 (orange) and the linker region (yellow).