The University of Douai was a former university in Douai, France. With a medieval heritage of scholarly activities in Douai, the university was established in 1559 and lectures started in 1562. It closed from 1795 to 1808. In 1887, it was transferred as University of Lille 27 km away from Douai.

Douai is a city in the Nord département in northern France. It is a sub-prefecture of the department. Located on the river Scarpe some 40 kilometres from Lille and 25 km (16 mi) from Arras, Douai is home to one of the region's most impressive belfries.