The Würzburg–Aschaffenburg railway is an 89 kilometre-long railway line in the Bavarian province of Lower Franconia. It runs from Würzburg via Gemünden (Main) to Aschaffenburg. It is particularly important for long-distance and goods traffic because it links the Rhine-Main conurbation immediately northwest of Aschaffenburg with the Lower Franconian city of Würzburg and beyond it to the metropoles of Nuremberg and Munich. The German name derives from the fact that it initially runs parallel to the River Main and then cuts through the Spessart hills. It was opened on 22 June 1854 by the Frankfurt-Hanau Railway Company and is one of the oldest railways in Germany.

Gemünden am Main is a town in the Main-Spessart district in the Regierungsbezirk of Lower Franconia (Unterfranken) in Bavaria, Germany and lies roughly 40 km down the Main from Würzburg. Gemünden has around 10,000 inhabitants.