Western blot
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The Western blot, or Western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract, and to vis
Western blot workflow
Western blot HIV test where the first two strips are negative and positive controls followed by actual tests
From gel to publication
Immunoelectrophoresis
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Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of immun
Western blot
…visualize. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds…
Crossed immunoelectrophoresis of 2 microlitres of normal human serum. The electrophoresis was performed in thin layers of agarose; the pictured gel is about 7x7 cm. The lower part is the first dimension gel without antibodies, where the serum was applied into the slot at the lower left. The upper part is the second dimension gel with Dako antibodies against human serum proteins. More than 50 major serum proteins can be named.
Fused rocket immunoelectrophoresis of an affinity chromatographic separation of human serum proteins on con A. A 15 microlitre sample from each fraction was applied (starting from left) and allowed to diffuse about one hour, then electrophoresis was performed overnight. Peak "a" was not retained, while peak "b" was retained and eluted with methyl-mannose. The arrow indicates some slightly retained proteins.
Plasmodium Glutamate dehydrogenase (pGluDH) separated by Counterimmunoelectrophoresis