The 29th Assembly District of Wisconsin is one of 99 districts in the Wisconsin State Assembly. Located in western Wisconsin, the district comprises all of Buffalo and Pepin counties, along with most of Pierce County, and much of Trempealeau County. It includes the cities of Alma, Arcadia, Blair, Buffalo City, Durand, Fountain City, Independence, Mondovi, Prescott, and Whitehall. The district also contains Trempealeau National Wildlife Refuge and Perrot State Park. The district is represented by Republican Clint Moses, since January 2021.

The Wisconsin State Assembly is the lower house of the Wisconsin Legislature. Together with the smaller Wisconsin Senate, the two constitute the legislative branch of the U.S. state of Wisconsin.