The 35th Assembly District of Wisconsin is one of 99 districts in the Wisconsin State Assembly. Located in northern Wisconsin, the district comprises all of Lincoln and Langlade counties, and parts of northern Oconto County, northwest Shawano County, southern Oneida County, and northern Marathon County. It includes the cities of Antigo, Merrill, and Tomahawk, and the villages of Aniwa, Eland, Maine, Mattoon, and White Lake. It also contains the Council Grounds State Park and about a quarter of the Nicolet National Forest. The district is represented by Republican Calvin Callahan, since January 2021.

The Wisconsin State Assembly is the lower house of the Wisconsin Legislature. Together with the smaller Wisconsin Senate, the two constitute the legislative branch of the U.S. state of Wisconsin.