Zymography
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Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography, in situ zymo
Hemoglobinase imprint of Plasmodium knowlesi.
Gel electrophoresis
Videos
Gel electrophoresis is an electrophoresis method for separation and analysis of biomacromolecules and their fragments, based on their size and charge through a gel. It is used in clinical chemistry to
Zymography
…Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography…
Gel electrophoresis apparatus – an agarose gel is placed in this buffer-filled box and an electric current is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the positively charged anode (red wire). This occurs because phosphate groups found in the DNA fragments possess a negative charge which is repelled by the negatively charged cathode and are attracted to the positively charged anode.
Inserting the gel comb in an agarose gel electrophoresis chamber
Specific enzyme-linked staining: Glucose-6-Phosphate Dehydrogenase isoenzymes in Plasmodium falciparum infected Red blood cells